Table 4. Glass forming properties of PG and glycerol VSs with 400μg/ml of AFP during cooling in the liquid nitrogen and warming in the original solution by cryoloop, cryotop and OPS methods.

Solution	Original Solution + 400μg/ml AFP
	OPS	Cryotop	Cryoloop
Glycerol VS1	VD	VD	VD
Glycerol VS2	VD	VD	VD
Glycerol VS3	C	VD	C
Glycerol VS4	C	VD	C
Glycerol VS5	C	VD	C
PG VS1	VV	VV	VV
PG VS2	VD	VV	VD
PG VS3	VD	VD	VD
PG VS4	C	VD	C
PG VS5	C	VD	C

VV—indicates vitrification when cooled as well as when warmed. VD—indicates vitrification when cooled but devitrifies upon thawing. C—indicates crystallization occurs when placed in liquid nitrogen.