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. 2015 May 26;10(5):e0126526. doi: 10.1371/journal.pone.0126526

Fig 4. Induction of PKA activity by TRAIL restrains its pro-apoptotic potential.

Fig 4

(A) Parental SW480 cells were stimulated for 30 minutes with forskolin and cell extracts were analyzed by immunoblot using selective PKA or PKC substrate antibodies as well as an anti-phospho-S133 CREB antibody. (B) Parental SW480 cells were analyzed as above after stimulation with TRAIL or FasL as indicated for 15 to 120 minutes. (C) Parental SW480 cells were pre-treated or not for 30 minutes with indicated concentrations of forskolin, followed by 6 hours stimulation with 100 or 500 ng/ml FasL or TRAIL. Apoptosis was quantified by Hoechst staining. (D) Parental SW480 cells were pre-treated or not for 30 minutes with 100 μM H89 or 20 minutes with 4 mM 8B, followed by 6 hours stimulation with 100 or 500 ng/ml TRAIL. (C and D) Data represent the mean ± SD of at least three different experiments. (**P<0.01; ***P<0.001 respective to control or H89 stimulated cells; ns stands for not statistically relevant).