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. 2015 May 26;10(5):e0127641. doi: 10.1371/journal.pone.0127641

Fig 2. EGR1-mediated repression of endogenous MEF2 activity in cardiomyocytes is dependent on MEF2 DNA binding.

Fig 2

Repression of MEF2-dependent costamere promoters by EGR1 requires intact MEF2 binding site. NRVMs were transduced with AdEGR1 and Adβgal at an MOI of 25. Twenty four hours post-transduction, 1.5 kb myomaxin-Luc and 1.5 kb myomaxinΔMEF2-Luc were transfected. Luciferase activity was measured 48 hrs after transfection. Firefly luciferase readings were normalized to Renilla luciferase readings. Data are mean ± SEM. ***p<0.001, n.s. not significant.