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. 2015 May 26;10(5):e0128239. doi: 10.1371/journal.pone.0128239

Fig 1. Regulation of HIF-1α protein expression independent of mRNA regulation.

Fig 1

A. Western blot of extracts from control and two different 22Rv1 and PC3 clones expressing ERβ2 assayed with HIF-1α antibody. B. Relative mRNA expression of ERβ2 and HIF-1α in the ERβ2clones of the 22Rv1 cell line. The graph shows the data as fold change compared with the control (mean of three separate experiments (±s.e.m.) calculated using Student’s t-test, *p≤0.016). C. Relative mRNA expression of HIF-1α in the ERβ2 #2 clone of the 22Rv1 cell line. The graph shows the data as fold change compared with the control (mean of three separate experiments (±s.e.m.) calculated using Student’s t-test, **p≤0.006).