Skip to main content
. 2015 May 26;10(5):e0128239. doi: 10.1371/journal.pone.0128239

Fig 3. Twist1 promoter with intact HIF-1α response element is activated by ERβ2 expression while the promoter with mutated site is not activated.

Fig 3

A. Description of Twist1 promoter constructs used. B. LNCaP cells transfected with Twist1-promoter-Luciferase reporter and increasing concentration of ERβ2 expression plasmid. The graph shows the data as an increase in luminescence of cells transfected with increased concentration of ERβ2 (mean of three separate experiments (±s.e.m.) calculated using Student’s t-test, **p≤0.0034). C. Transfection of Twist1-promoter-Luciferase reporter with (Twist) or mutated (mTwist) HIF-1α response element together with expression plasmids for HIF-1α or ERβ2 in LNCaP cells. The graph shows the data as an increase in luminescence of cells transfected with increased concentration of ERβ2 (mean of three separate experiments (±s.e.m.) calculated using Student’s t-test, ****p≤0.0001, ###p≤0.002).