Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 26;10(5):e0128239. doi: 10.1371/journal.pone.0128239

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Dey et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 4 — A. His-tagged LBD-ERβ2 pull down with nickel agarose beads of in vitro translated HIF-1α compared to His-tagged LBD-ERα, His-tagged LBD-ERβ1, His-tagged LBD-ERβ2 and His-tagged ERβ2ΔCX TNT is lane with only coupled reticulocyte lysate. B. Expression of the biotin ligase BirA, HA-HIF-1α, and the N-terminal biotin consensus peptide fused receptor constructs B7TEV-ERα, B7TEV-ERβ1, B7TEV-ERβ2, B7TEV-ERβ5, and B7TEV-ERβΔCX in PC3 cells. Cell extracts were then subjected to pulldown with streptavidin magnetic beads and proteins separated by SDS-PAGE followed by detection with HIF-1α antibody.