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. 2015 May 26;10(5):e0127687. doi: 10.1371/journal.pone.0127687

Fig 4. Time course of HTT isoform expression in hESCs differentiating to telencephalic neural fate.

Fig 4

(A-D) RUES2 hESCs were differentiated to neural fate by blocking both branches of TGFβ signaling (default mechanism) as described in Materials and Methods. Values are normalized by GAPDH and displayed as fold change to day 0 values. Only the HTT-Δ10 isoform consistently decreases as the cells differentiate, while all three other isoforms maintain their expression levels unchanged. Error bars represent the standard error of the mean of 3 to 6 independent replicates. * p<0.05 vs d0.