Table 2. DNA restriction-modification genes in E. coli K-12 MG1655. a .
Gene | Product | Activity | |
---|---|---|---|
DNA MTases | |||
dam | orphan MTase M.EcoKDam | Gm6ATC | |
dcm | orphan MTase M.EcoKDcm | Cm5CWGG | |
yhdJ | orphan MTase M.EcoKII | ATGCm6AT (silent) | |
hsdM | Type I MTase M.EcoKI | Am6ACNNNNNNGTGC | |
Other Genes | |||
hsdR | Type I restriction ENase R.EcoKI | ||
hsdS | Type I specificity subunit S.EcoKI | ||
mcrA | Type IV restriction ENase | ||
mcrBC | Type IV restriction ENase | ||
mrr | Type IV restriction ENase |
a All of these genes have been deleted or otherwise inactivated in ER2796 except for yhdJ, which is additionally inactivated in ER3413.