Fig 6. Effect of DNase co-treatment with antimicrobials on the MAH A5 biofilm.

Biofilms were formed in HBSS in 96-well plates and incubated at 22°C and 37°C for 7 days. Supernatants were gently removed and replaced with HBSS containing 16 μg/ml of (A) moxifloxacin or (B) clarithromycin further supplemented 1x DNase buffer ± 100 Kunitz units per ml of recombinant DNase. All biofilms (including 22°C) were incubated for 48 hours at 37°C. Wells were mixed 50 times via pipetting to remove attached bacteria and samples were diluted and plated to obtain total viable bacteria. For both A and B, bars represent the average of 4 wells of biofilm processed independently ± standard deviation. Data shown are representative of three biological replicates. Statistical comparisons: ** P < 0.01; *** P < 0.001.