Fig 1. Detection of autoantigens with sera from KD patients.

(A) 2-D western blot analyses were performed as described under Materials and Methods, and a typical result obtained using sera from a KD patient is shown. Western blot signals detected using ECL plus are shown by green pseudocolor and total protein profile detected using staining with Cy5 dye is shown by the red pseudocolor, and the two signals were merged. Arrows demonstrate spots that were detected with sera from multiple KD patients. Each serum was analyzed 3–5 times. (B) A magnified view of the area containing the detected spots (indicated by a white box in (A)) is shown for the same patient and for an antibody-negative patient.