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. 2015 May 26;10(5):e0128189. doi: 10.1371/journal.pone.0128189

Fig 4. Specificity of an anti-TMABA-DH antibody.

Fig 4

siRNA treatment of HUVEC significantly decreased the levels of TMABA-DH mRNA detected by RT-PCR and agarose gel electrophoresis (A) and protein in immunoblot analysis (B), and antibody reactive signals using immunohistochemistry (C), as compared with control or scrambled siRNA (siRNA sc.) treatment. In (C), nuclei were stained with DAPI (Blue). In (D), HUVEC were immunostained with the antibody (green) or control IgG with nuclei being stained with DAPI (blue). Bars indicate 50 μm. The experiments were repeated 3 times.