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. 2015 May 26;10(5):e0127319. doi: 10.1371/journal.pone.0127319

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© 2015 Orhan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) Target and sequence of primers and probes used for qPCR, FAM: 6-carboxyfluorescein and TAM: tetramethylrhodamine. (B) WT endogenous rat Rho (light grey) and P23H mutant mouse Rho (dark grey) copy numbers were evaluated by qPCR and 2^-ΔΔCt method for WT (WT), hemizygous P23H-1 (P23H hemi) and homozygous P23H-1 (P23H homo) rats. All experiments were conducted on the gDNA of 5 rats per group. Three asteriks (***) indicate a p value of <0.001, error bars represent standard errors.