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. 2015 May 26;10(5):e0127319. doi: 10.1371/journal.pone.0127319

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© 2015 Orhan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Target and sequence of primers used for RT-qPCR. (B) WT endogenous rat Rho (light grey) and P23H mutant mouse Rho (dark grey) relative expressions were evaluated by qPCR and 2^-ΔΔCt method for 1-month-old WT (WT) and hemizygous P23H-1 (P23H hemi) rat retinas. In 1-month-old WT rats endogenous Rho expression was assessed at 100%. All experiments were conducted on the retinal cDNA of 5 rats per group. Three asteriks (***) indicate a p-value of <0.001, error bars represent standard errors.