Fig 1. Effects of ZnCl₂ on Fluo-3 fluorescence in C6 glioma cells.

(A) C6 glioma cells were cultured for 24 h, followed by loading of Fluo-3 in the presence of CaCl₂ and subsequent determination of the fluorescence intensity in either the presence or absence of ZnCl₂ every 1 min for 21 min. (B) Cells were loaded with Fluo-3 in either the presence or absence of CaCl₂, followed by determination of the fluorescence intensity in the presence of ZnCl₂ every 1 min. Values are the mean±S.E. of the rate of fluorescence change in 3 different experiments.