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. 2015 May 26;10(5):e0127421. doi: 10.1371/journal.pone.0127421

Fig 10. Effects of TPEN on Fluo-3 and Rhod-2 fluorescence in HEK293 cells with acquired NMDAR channels.

Fig 10

HEK293 cells were transfected with expression vectors of GluNR1 and GluNR2A, followed by further culture for an additional 24 h and subsequent loading of either (A) Fluo-3 or (B) Rhod-2 in the presence of Gly. Cells were then exposed to Glu in either the presence or absence of TPEN during the determination of each fluorescence intensity every 1 min. Values are the mean±S.E. of the rate of fluorescence change in 3 different experiments.