Skip to main content
. 2015 May 26;10(5):e0127421. doi: 10.1371/journal.pone.0127421

Fig 13. Effects of ZnCl2 on PI and Hoechst33342 staining in C6 glioma cells.

Fig 13

Cells were exposed to ZnCl2 at concentrations of 0.1 to 1 mM in the presence of CaCl2 for 1 h, followed by culture for an additional 24 h and subsequent double staining with PI and Hoechst33342 for nuclear DNA. Typical micrographs are shown in the panel (A), while in the panel (B) value are the mean±S.E. of percentages of PI-positive cells over Hoechst33342-positive cells in 3 independent experiments. *P<0.05, **P<0.01, significantly different from the control value in cells not exposed to ZnCl2.