Fig 1. The first AUG codon in mouse Nelf-b gene is dispensable for full-length protein translation.

(A) Diagram showing various NELF-B expression constructs. “AUG” contains the NELF-B coding sequence starting at the annotated initiating AUG. The solid box denotes the location of the Flag peptide. “FL” contains the entire 5’-UTR sequence of Nelf-b. The “CUC” construct has the mutation in the annotated AUG codon. (B) NELF-B and Flag western blot showing the expression of the constructs described in (A) in 293T cells. Two independent clones of “FL-CUC” and “Flag-FL-CUC” were included.