Table 1.
Crystallographic data and refinement statistics
| Native GFP-APC3Δloop/APC16C |
SeMet GFP-APC3ΔloopΔC20/APC16C |
Native GFP-APC3Δloop |
|
|---|---|---|---|
| Data Collection | |||
| Beam line | NECAT 24-ID-C | NECAT 24-ID-E | NECAT 24-ID-C |
| Space Group | P43 | P43 | P65 |
| Unit cell parameters | |||
| a, b, c (Å) | 116.8, 116.8, 185.1 | 116.0, 116.0, 184.3 | 118.4, 118.4, 273.7 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 90, 90, 120 |
| Resolution (Å) (Highest shell) | 50 – 3.3 (3.42 – 3.3) | 50 – 3.25 (3.39 – 3.25) | 50 – 4.25 (4.40 - 4.25) |
| Wavelength (Å) | 1.07160 | 0.97918 | 0.97920 |
| Number of measured reflections | 254827 | 162277 | 89519 |
| Number of unique refections | 37171 | 38356 | 15458 |
| Overall Rsym | 0.100 (0.829) | 0.125 (0.760) | 0.119 (0.826) |
| Completeness (%) | 100.0 (100.0) | 99.9 (100.0) | 99.9 (100.0) |
| Overall I/σI | 23.7 (3.5) | 12.8 (2.3) | 17.2 (2.5) |
| Multiplicity | 6.9 | 4.2 | 5.8 |
| Refinement | |||
| Resolution (Å) | 50 - 3.3 | 49.94 – 3.25 | 49.67 – 4.25 |
| Rwork/Rfree | 0.2033/0.2441 | 0.2097/0.2485 | 0.2548/0.2823 |
| rmsd bond lengths (Å) | 0.006 | 0.006 | 0.005 |
| rmsd bond angles (°) | 1.039 | 1.065 | 1.243 |
| Number of atoms | |||
| Proteins | 7598 | 7542 | 6066 |
| Ramachandran statistics | |||
| Preferred (%) | 93.9 | 93.6 | 94.2 |
| Allowed (%) | 6.1 | 6.4 | 5.6 |
| Disallowed (%) | 0 | 0 | 0.2 |