Figure 4.
Effect of the ASK1 inhibitor on JNK activation. Western blot analysis (A) of p-JNK and total JNK in the cytosolic fraction of livers from untreated mice (Ctrl), mice treated with 30 mg/kg ASK1 inhibitor or mice treated with the same volume of vehicle (55% PEG in water) 30 min prior to 300 mg/kg of APAP administration. Liver samples were obtained 6 or 24 h after APAP treatment. Densitometric quantification was performed on bands of p-JNK (B) and total JNK (C). Data represent means ± SE of n=4 animals. *P<0.05 compared with control groups. #P<0.05 compared with APAP+Vehicle group.