Fig. 1.

Outline of the protocols for the experiments for histopathology of the colon. Experiment 1 consisted of DSS and BP/DSS groups, and the mice in these groups (6 to 12 mice per group) were necropsied at weeks 14 and 17. In this report, the first week of the experiment is defined as week 0 (indicated as W0 in these figures). Experiment 2 consisted of vehicle, BP, DSS, and BP/DSS groups, and all mice were necropsied at weeks 4, 7, 9 and 11 (6 or 8 mice/group). Male CD2F₁ mice were treated with BP at an oral dose of 125 mg/kg/day for 5 consecutive days, and starting 10 days after the last dose, mice were given 4% DSS in drinking water for 1 week once (at week 2) or twice (intermittently, at weeks 2 and 5 separated by a 2-week DSS treatment free interval period). Salad oil and distilled water were used as vehicle controls for BP and DSS solutions, respectively.