Figure 1.

Spinal inhibition of PKCθ, but not other isoforms, blocks pLTF. A, Representative neurograms after AIH from rats given intrathecal injections (12 μl) of PKC inhibitors. ↓ indicates intrathecal delivery of inhibitor. Dotted line indicates baseline amplitude. Gray represents increases from baseline. B, Averaged percentage increase from baseline for each group at 60 min after AIH. Delivered drug doses: vehicle, n = 20; 1–2 mm Gö 6983, n = 8; 5 mm NPC-15437 (NPC), n = 6; 280 μm BIS, n = 5; 2–20 μm sotrastaurin, n = 5; myristolated pseudosubstrate peptide targeting PKCθ (theta inhibitory peptide, TIP, 2 μg/μl), n = 5. C, Average pLTF following intrathecal NPC or TIP. ^#p < 0.05 versus time controls (no AIH). *p < 0.05 versus vehicle.