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. 2015 May 27;35(21):8359–8375. doi: 10.1523/JNEUROSCI.2850-14.2015

Figure 8.

Figure 8.

ARNO-E156K significantly increased integrin level in axons as well as axon length. A, In day 9 cortical neurons transfected with GFP, integrin β1 was absent in axons beyond the AIS (stained with AnkG). B, Overexpression of ARNO-E156K increased β1 integrin level in axons including in the distal part (ii,iii). C, Combination of ARNO-E156K with taxol treatment showed a similar effect. D, Integrin β1 fluorescent intensity in axons is increased with ARNO-E156K or the combination treatment (ARNO-E156K vs GFP: 3.168 ± 0.362; ARNO-E156K + taxol vs GFP: 6.223 ± 1.436; p < 0.001 in both cases). Compared with ARNO-E156K alone, the combination increased integrin β1 in the proximal 200 μm (t = 0.0027, paired t test), but not in the distal part. E, ARNO-E156K significantly increased axon length; combination with taxol had no additional effect. ***p < 0.001. Scale bars in A–C, 50 μm.