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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: Cold Spring Harb Protoc. 2015 Mar 2;2015(3):272–275. doi: 10.1101/pdb.prot085084

FIGURE 1.

FIGURE 1

A single-cell invasive growth assay. Cells grown on synthetic media lacking glucose undergo filamentous growth within the first few cell divisions. Round mother cells (M) produce elongated progeny (D1, D2, and D3) that propagate away from the mother (D2A, D1A, D1B, and D1A1). Bar, 5 μm. Spotting glucose (GLU) at the center of the plate rescues the round cell shape and axial budding pattern.