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. 1973 May;3(5):590–598. doi: 10.1128/aac.3.5.590

Double-Stranded Ribonucleic Acid from Mengo Virus: Production, Characterization, and Interferon-Inducing and Antiviral Activities in Comparison with Polyriboinosinic · Polyribocytidylic Acid

E Falcoff 1,2, R Falcoff 1,2, J Cherby 1,2, J Florent 1,2, J Lunel 1,2, L Ninet 1,2, Y De Ratuld 1,2, R Tissier 1,2, B Vuillemin 1,2, G H Werner 1,2
PMCID: PMC444463  PMID: 4364180

Abstract

Mengo virus double-stranded ribonucleic acid (dsRNA) was obtained on a semi-industrial scale from infected cultures of BHK-21 cells grown in suspension. Yield of the extraction and purification operations was small (about 22 mg from 1011 cells in a 100-liter culture). Physicochemical characterization of this dsRNA gave an estimated molecular weight close to 4 × 106, a density of 1.59 (similar to that of the poliovirus dsRNA), and a thermal transition midpoint of 94 C. This product was a little more toxic for the mouse, by the intravenous route, than polyriboinosinic · polyribocytidylic acid (poly I:C) and strictly comparable in this respect to poliovirus dsRNA. The interferon-inducing capacity in the mouse and the antiviral activities in the mouse (infected with encephalomyocarditis, Semliki Forest, influenza, foot-and-mouth disease, and murine hepatitis viruses) and in the rabbit (Shope fibroma virus) of the ultraviolet light-inactivated product were practically identical, on a quantitative basis, with those of poly I:C. In vitro and in vivo experiments showed the dsRNA from Mengo virus to be slightly but significantly more resistant than poly I:C to the inactivating effect of human serum.

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Selected References

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