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. 2015 May 27;6:357. doi: 10.3389/fpls.2015.00357

Figure 2.

Figure 2

Sensitivity of the atpolλ-1 mutant to various DNA damages. Sterilized wild-type and atpolλ-1 seeds were sown on solid MS agar containing methylmethano sulfate (A) mitomycin C (B), or methyl viologen (C), and root length was measured after 7 days. The 3 day-old wild-type and atpolλ-1 seedlings grown on MS plates were exposed to UV-B, and then grown for two more days under normal light (D) or orange light (E). Sterilized wild-type and atpolλ-1 seeds were γ-irradiated and sown on MS-agar plates. Root length was measured after 7 days (F). Error bars represent the standard error of the mean of three independent experiments with 24–32 seedlings (average n = 30) for 2A, with 23–36 seedlings (average n = 28) for 2B, with 15–32 seedlings (average n = 25) for 2C, with 13–23 seedlings (average n = 20) for 2D, with 12–19 seedlings (average n = 15) for 2E, and with 18–48 seedlings (average n = 28) for 2F per line, per concentration, per replicate plates. The difference in relative root growth between wild type and atpolλ-1 mutants was significant at 120 Gy in Panel (F) (*p < 0.05, t-test).