Figure 7.

Transcriptional responses of DSB repair genes in wild-type and mutants in response to DSBs. Expression levels of (A) AtBRCA1, (B) AtKu80, and (C) DNA ligases (AtLig1, AtLig4, and AtLig6) after BLM treatment. Five day-old wild-type and mutant seedlings grown on MS agar plates were embedded in water containing 30 μg mL⁻¹ BLM for 1 h. Followed by rinse with sterilized water, BLM-treated seedlings were transferred onto MS agar plates before collection at each time point after treatment. All values were normalized to the expression level of control genes, and then relative expressions compared to untreated controls were calculated. Error bars indicate the standard error of the mean. ^*Significant at P < 0.05; ^**significant at P < 0.01.