Figure 8.

Transformation efficiency in wild-type and DSB-deficient mutants. The pFLAR101 vector was transformed into T₀ plants through Agrobacterium-mediated floral dipping and T₁ seeds were harvested as described in Materials and Methods. The T₁ transformed seeds overexpressing the DsRED protein were selected by fluorescence microscopic observation and transformation efficiency was calculated based on the number of fluorescent seeds. Each rhombus represents transformation efficiency per line per trial. Transformation experiments were repeated three times.