Table 2.
Fatty acids | Microwave assisted extraction with hexane | Microwave assisted extraction with chloroform | Ultrasound assisted extraction with hexane | Ultrasound assisted extraction with chloroform |
---|---|---|---|---|
C10:0 | 0.64 ± 0.00a | 0.85 ± 0.00b | 0.69 ± 0.01c | 0.95 ± 0.01d |
C12:0 | 0.35 ± 0.00a | 1.29 ± 0.01b | 1.92 ± 0.01c | 1.04 ± 0.01d |
C14:0 | 0.35 ± 0.00a | 0.46 ± 0.01b | 0.37 ± 0.00c | 0.41 ± 0.01d |
C14:1 | 0.08 ± 0.00a | 0.42 ± 0.01b | 0.17 ± 0.00c | 0.26 ± 0.00d |
iso–C15:0 | 0.13 ± 0.00 | – | – | – |
C15:0 | 1.72 ± 0.00a | 1.65 ± 0.01b | 1.68 ± 0.01c | 1.63 ± 0.01b |
C15:1ω–5 | 0.12 ± 0.00a | 0.25 ± 0.00b | 0.10 ± 0.00c | 0.17 ± 0.00d |
C16:0 | 10.80 ± 0.03a | 11.38 ± 0.05b | 11.07 ± 0.04c | 10.74 ± 0.03a |
iso–C16:0 | 0.45 ± 0.00a | 0.46 ± 0.00a | 0.44 ± 0.01b | 0.43 ± 0.00b |
C16:1ω–9 | 0.65 ± 0.00a | 0.56 ± 0.00b | 0.67 ± 0.01a | 0.62 ± 0.01c |
iso–C17:0 | 0.18 ± 0.00a | 0.27 ± 0.00b | 0.14 ± 0.00c | 0.16 ± 0.00d |
cyclo–C17:0 | 1.32 ± 0.01a | 1.27 ± 0.01b | 0.86 ± 0.01c | 0.95 ± 0.01d |
C17:0 | 0.88 ± 0.01a | 0.54 ± 0.01b | 0.82 ± 0.01c | 0.69 ± 0.01d |
C17:1ω-7 | 0.43 ± 0.00a | 0.48 ± 0.00b | 0.45 ± 0.00c | 0.47 ± 0.00d |
C18:0 | 1.63 ± 0.02a | 1.95 ± 0.02b | 1.89 ± 0.02c | 2.04 ± 0.03d |
C18:1ω-9 | 13.91 ± 0.14a | 13.92 ± 0.13a | 14.22 ± 0.11b | 14.38 ± 0.16b |
C18:1ω-7 | 0.54 ± 0.00a | 0.48 ± 0.00b | 0.52 ± 0.02a | 0.55 ± 0.01a |
C18:2ω-6 | 57.00 ± 0.56a | 56.12 ± 0.75a | 56.10 ± 0.71a | 56.45 ± 0.59a |
C18:2cis–9trans-11 | 0.58 ± 0.00a | 0.57 ± 0.00b | 0.54 ± 0.01c | 0.56 ± 0.01cb |
C18:3ω-6 | 0.56 ± 0.00a | 0.33 ± 0.00b | 0.48 ± 0.00c | 0.42 ± 0.00d |
C18:3ω-3 | 0.09 ± 0.00a | 0.13 ± 0.00b | 0.08 ± 0.00c | 0.10 ± 0.00d |
C18:4ω-3 | 0.09 ± 0.00 | - | - | - |
C20:0 | 0.16 ± 0.00a | 0.19 ± 0.00b | 0.15 ± 0.00c | 0.18 ± 0.00d |
C20:1ω-9 | 0.09 ± 0.00a | 0.12 ± 0.00b | 0.10 ± 0.00c | 0.11 ± 0.00d |
C20:2ω–6 | 0.30 ± 0.00a | 0.36 ± 0.00b | 0.35 ± 0.01bc | 0.34 ± 0.01c |
C20:5ω–3 | 0.09 ± 0.00a | 0.17 ± 0.00b | 0.09 ± 0.00a | 0.17 ± 0.00b |
C22:5ω–6 | 1.10 ± 0.01a | 1.04 ± 0.01b | 1.24 ± 0.01c | 1.13 ± 0.01d |
C22:5ω-3 | 0.91 ± 0.00 | – | – | – |
C24:0 | 2.03 ± 0.04a | 1.86 ± 0.02b | 2.23 ± 0.02c | 2.15 ± 0.02d |
C22:6ω–3 | 1.62 ± 0.03a | 1.27 ± 0.01b | 1.51 ± 0.02c | 1.34 ± 0.02d |
C24:1ω–9 | 1.20 ± 0.03a | 1.62 ± 0.01b | 1.12 ± 0.03c | 1.56 ± 0.01d |
Σω:0 (SFA) | 20.64 ± 0.12a | 22.18 ± 0.12b | 22.26 ± 0.14b | 21.37 ± 0.15c |
Σω:1 (MUFA) | 17.02 ± 0.17a | 17.85 ± 0.15b | 17.35 ± 0.13a | 18.12 ± 0.14b |
Σω:n (PUFA) | 62.34 ± 0.61a | 59.98 ± 0.57b | 60.39 ± 0.46b | 60.51 ± 0.52b |
Σω:3 | 2.80 ± 0.04a | 1.57 ± 0.02b | 1.68 ± 0.04c | 1.61 ± 0.03bc |
Σω:6 | 59.53 ± 0.57a | 58.41 ± 0.56a | 58.71 ± 0.49a | 58.90 ± 0.52a |
ω-6/ω-3 | 21.24 ± 0.08a | 37.21 ± 0.19b | 34.95 ± 0.15c | 36.58 ± 0.17d |
PUFA/SFA | 3.02 ± 0.01a | 2.70 ± 0.01b | 2.71 ± 0.01b | 2.83 ± 0.01c |
MUFA/SFA | 0.82 ± 0.00a | 0.80 ± 0.00b | 0.78 ± 0.01b | 0.85 ± 0.01c |
UFA/SFA | 3.84 ± 0.35a | 3.47 ± 0.31a | 3.49 ± 0.28a | 3.68 ± 0.36a |
C18:2ω-6/C18:1ω-9 | 4.14 ± 0.00a | 4.34 ± 0.00b | 3.95 ± 0.02c | 3.93 ± 0.02c |
Values are mean ± SD calculated from three different experiments performed in triplicate. Means with different letters in the same column are significantly different (p<0.05