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. 2014 Aug 6;52(6):3158–3168. doi: 10.1007/s13197-014-1487-3

Table 1.

Optimized polyphenol extraction strategies

Tea biomolecule Extraction condition Reference
Polyphenols content-in-extracted solids (PES) in black tea 50:1 water-to-tea ratio and 40 min Chandini et al. 2011
Catechins in Green Tea Leaves 2 h room temperature extraction using a 2 % phosphoric acid-40 % etoh solution Choung and Lee 2011
Selective extraction and preconcentration of epicatechin Solid-phase extraction (SPE) by molecularly imprinted polymer prepared from acrylamide monomer Ding et al. 2006
Catechin extraction from milk tea beverage (Brewed green tea combined with skim milk) Use of pepsin (40.0 mg/ml) Ferruzzi and Green 2006
Polyphenol from yellow tea Extraction with ultrasound probe than ultrasonic bath in 75 % aqueous ethanol medium Horžić et al. 2012
Total polyphenol (TP) 50 % (ml/ml) of ethanol concentration, 20:1 (ml/g) of liquid/solid ratio and 500mpa of high hydrostatic pressure for 1 min by high hydrostatic pressure extraction (HHPE) Jun et al. 2009
Major catechins from green tea 50 % (v/v) ethanolic solvent extraction, solvent to tea ratio 20 ml:1 g, 400mpa pressure for 15 min by ultrahigh pressure extraction Jun et al. 2010
Two scale extraction for production of catechin- enriched tea drinks Brewing green tea at 1:50 tea/water ratio in water thermostated at 30 °C for 30 min followed by water extraction of leaves by squeezing and then second brewing step in water maintained at 75 °C for 40 min Labbé et al. 2008; Bazinet et al. 2007
The first brewing step at 50 °C for 10 min. Followed by water extraction of leaves by squeezing and then soaked for 10 min, for the second brewing step, at 80 °C water. The Two-step extraction procedure was repeated three times
Extraction of catechin from Longjing tea 400 W, 1 min irradiation duration, 1:15 gm/ml tea to water ratio by Microwave assisted extraction Li et al. 2010
Total catechins 50 % (v/v) ethanol for 10 min for dry tea, and 75 % (v/v) ethanol for 10 min for fresh tea leaf. For commercial purpose water mediated extraction at 80 °C Liang et al. 2007
Tea polyphenols Membrane separation by nanofiltration with G-membrane in 80 % of ethanol solution Nwuha 2000
Tea catechin 4 min microwave-assisted time, 50 % (v/v) ethanol concentration in water, liquid/solid ratio of 20:1 (ml/g) and pre leaching time of 90 min by microwave assisted extraction Pan et al. 2003
Aqueous extaction of catechins Either high temperature (95 °C) and short extraction time (5–10 min), or lower temperature (60 or 80 °C) and longer extraction time (20 min) to avoid catechin degradation and ratio of solvent to material (100 ml:1 g) or lower ratios (40 ml:1 g or 9 ml:1 g) and a multi-step extraction procedure to obtain more catechin Perva-Uzunalic et al. 2006
Polyphenol extraction from white and black tea Accelerated phenolic extraction from green tea by lemon juice addition in water extract, for prolonged extraction of catechin with aqueous ethanol (40 %) Rusak et al. 2008
Epicatechin (EC), epicatechin Gallate(ecg), epigallocatechin (EGC), and epigallocatechin gallate(egcg), the major four catechin from Hawaii tea leaves Increase in content of four catechins with increasing leaf age, moving from the bud to first and second leaves Song et al. 2012
Concentration of tea polyphenols by molecular distillation and drying by spray drying 70 °C distillation temperature, 10 ml/min flux, and 1,200 n/min rotational speed for concentration and 170 °C distillation temperature, 3 ml/min feed flux, 30 % feed concentration, and 30 m3/h wind capacity for drying Tang et al. 2011
Catechin by water extraction 80 °C for 30 min, a tea particle size of 1 mm, a brewing solution ph <6 and a tea to- water ratio at 50:1 (ml/g) for maximal extraction and tea to- water ratio at 20:1 (ml/g) for cost efficiency along with maximal yield Vuong et al. 2011b
Total polyphenol 60 % ethanol concentration, solid-to-liquid ratio 1∶ 12 g/ml at 80 °C, 10 min under the microwave power of 600 W. Wang et al. 2010
Catechins From green tea Ethanol concentration, 50 %; pressure, 490 mpa; and liquid/solid ratio, 20 ml/g by ultrahigh-pressure extraction Xu et al. 2012
Polyphenols From Iranian tea 20–40 min and 80 °C for EGC, EC and 80 min and 90 °C for catechin, EGC and EGCG Ziaedini et al. 2010
EC, ECG, EGC, and EGCG 7 min of steeping at a constant 100 °C at pH 3 Zimmermann and Gleichenhagen 2011