| Nutrient agar media composition | Heart infusion agar media Composition | ||
| Ingredients | g/L | Ingredients | g/L |
| Peptic digest of animal tissue | 5.0 | Calf brain, infusion form | 200.0 |
| Sodium chloride | 5. 0 | Beef heart, infusion form | 250.0 |
| Beef extract | 1.5 | Proteose peptone | 10.0 |
| Yeast extract | 1.5 | Dextrose | 2.0 |
| Agar | 15.0 | Sodium chloride | 5.0 |
| Final pH (at 25°C) | 7.4 ± 0.2 | Disodium phosphate | 2.5 |
| Agar | 15.0 | ||
| Final pH (at 25°C) | 7.4 ± 0.2 | ||
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