Table 2.
Discrimination of rice cultivars by DNA markers
Marker type | Fragment separation method | DNA Markers | Rice Cultivars | Reference |
---|---|---|---|---|
RFLP | Agarose | 43 RFLP probes | Indica and Japonica varieties | Zhang et al. (1992) |
RAPD | Agarose | OPA 13, OPA 16, OPA 17, OPA 19, OPB 8, OPB11, OPB 13, OPB 18, OPN 1 and OPN 17 | Basmati varieties | Choudhury et al. (2001) |
Microsatellites | Slab-gel | RM1,RM19,RM208,RM55,RM201,RM219,RM225,RM229,RM202,M2 and M16 | Basmati varieties | Bligh (2000) |
Microsatellites | Slab-gel | RM163, RM171, RM72, RM238, RM102, RM16, RM302, RM330 and RM16 | Basmati varieties | Nagaraju et al. (2002) |
Microsatellites | Agarose | RM1, RM21, RM38, RM170, RM210, RM226 and RM229 | Basmati varieties | Siwach et al. (2004) |
Microsatellites | Slab-gel | RM1, RM5, RM103, RM135,RM171, RM174, RM222 and RM252 | Basmati varieties | Jain et al. (2004) |
Microsatellites | Polyacrilamide gel | RM1,RM5,RM44,RM110,RM229, RM234,RM171,RM242 and RM255 | Basmati varieties | Pal et al. (2004) |
Microsatellites | Capillary electrophoresis | RM1,RM171,RM202,RM44,RM55,RM348,RM72 and RM241 | Basmati varieties | Archak et al. (2007) |
Microsatellites | Agarose, Slab-gel and Capillary electrophoresis | RM1,RM171,RM202,RM44,RM55,RM348,RM72 and RM241 | Basmati varieties | Vemireddy et al. (2007) |
Microsatellites | Agarose | RM11/147 | BR-11 | Rahman et al. (2007) |
RM151/289 | Badshabhog | |||
RM153/178 | BR-19 | |||
Microsatellites | Agarose | RM70, RM334, RM475, RM219, RM206, RM336, RM547, RM164, RM335, and RM276 | Parental lines and hybrids and some popular varieties | Sundaram et al. (2008) |
Microsatellites | Agarose | RM 206, RM 216, RM 258 and RM263 | Hybrid purity | Nandakumar et al. (2004) |
Indels | Agarose | R4M17,R4M43,R4M50,R5M13,R5M30,R6M14,R10M30,R11M23 and R12M27 | Basmati varieties | Steele et al. 2008 |
SCAR | Agarose | B1,B7,B43,B43a,E30,F6,G22,M2CG,M11,S13 and Wka9 | Koshihikari in processed rice products | Ohtsubo and Nakamura (2007) |
SNP | Dot-blot | 8 SNPs | 43 rice cultivars | Shirasawa et al. (2006) |
STS | Agarose | A7, B43, M11 and G22 | Cultivars of rice wine | Ohtsubo et al. (2008) |
STS | Agarose | PRR | Rice specific primer | Nakamura and Ohtsubo (2010) |