Table 3.
Methods used for quantification of the adulterants
Marker type | Fragment separation method | DNA Markers | Method of quantification | Reference |
---|---|---|---|---|
Microsatellites | Slab-gel | RM1,RM19,RM208,RM55,RM201,RM219,RM225,RM229,RM202,M2 and M16 | Calibration curve | Bligh (2000) |
Microsatellites | Capillary electrophoresis | RM1,RM171,RM202,RM44,RM55,RM348,RM72 and RM241 | Calibration curve-Logistic model | Archak et al. (2007) |
Real time PCR | Fgr and RM348 | Cycle number | ||
Microsatellites | Slab-gel, Capillary electrophoresis | RM1,RM171,RM202,RM44,RM55,RM348,RM72 and RM241 | Ratio of peak area of adulterant and total peak area | Vemireddy et al. (2007) |
Microsatellites | Slab-gel | RM222 | Calibration curve-Linear regression | Colyer et al. 2008 |
Microsatellites and Indel | Real time PCR | RM241 and BAD2 gene | High resolution melting profile | Ganopoulos et al. (2011) |
SCAR | Real time PCR | Wka9q, S13q, E30q, and F6q | Standard curve- Cycle number | Okunishi et al. (2005) |
Gene specific primer | Real time PCR | gos9 sequence | Cycle number | Herńandez et al. (2005) |