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. 2014 Sep 26;52(6):3187–3202. doi: 10.1007/s13197-014-1579-0

Table 3.

Methods used for quantification of the adulterants

Marker type Fragment separation method DNA Markers Method of quantification Reference
Microsatellites Slab-gel RM1,RM19,RM208,RM55,RM201,RM219,RM225,RM229,RM202,M2 and M16 Calibration curve Bligh (2000)
Microsatellites Capillary electrophoresis RM1,RM171,RM202,RM44,RM55,RM348,RM72 and RM241 Calibration curve-Logistic model Archak et al. (2007)
Real time PCR Fgr and RM348 Cycle number
Microsatellites Slab-gel, Capillary electrophoresis RM1,RM171,RM202,RM44,RM55,RM348,RM72 and RM241 Ratio of peak area of adulterant and total peak area Vemireddy et al. (2007)
Microsatellites Slab-gel RM222 Calibration curve-Linear regression Colyer et al. 2008
Microsatellites and Indel Real time PCR RM241 and BAD2 gene High resolution melting profile Ganopoulos et al. (2011)
SCAR Real time PCR Wka9q, S13q, E30q, and F6q Standard curve- Cycle number Okunishi et al. (2005)
Gene specific primer Real time PCR gos9 sequence Cycle number Herńandez et al. (2005)