Table 4.
Rf-values and color of spots of the chloroform fraction
| Extracts | Without revelation a | With revelation | Identified compounds | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NH3 A | Liebermann-Burchard B | FeCl3 C | KOH D | (CH3CO2)2Pb E | ||||||||||||||
| Rf | Visible | UV/366 nm | Rf | Visible | UV/366 nm | Rf | Visible | UV/366 nm | Rf | Visible | Rf | Visible | UV/366 nm | Rf | Visible | UV/366 nm | ||
| CF | 0.00 | Yellow | Yellow | 0.00 | Yellow | Yellow | 0.00 | Green | Yellowg | – | – | 0.00 | Brown | Yellow | 0.00 | Yellow | Yellow | Steroids B (Mamyrbekova-Bekro et al. 2013b; Kabran et al. 2011; Lagnika 2005) |
| 0.07 | – | Redl | 0.04 | – | Violet | – | – | – | – | – | – | – | – | 0.06 | – | Violet | Anthraquinone a (Mamyrbekova-Bekro et al. 2013b) | |
| – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0.12 | – | Violetd | Unidentified | |
| 0.70 | – | Violetd | – | – | – | 0.89 | Violet | Violet | – | – | – | – | – | 0.75 | – | Violetd | Steroidal saponin B (Mamyrbekova-Bekro et al. 2013a; Guy et al. 2010) | |
Mobile phase : Cyclohexan: AcOEt (10: 8 (v/v))
l Light, d Dark, g Greenish