Table 5.
Rf-values and color of spots of the ethyl acetate fraction
| Extracts | Without revelation a | With revelation | Identified compounds | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NH3 A | KOH B | AlCl3 C | FeCl3 D | ||||||||||||
| Rf | Visible | UV/366 nm | Rf | Visible | UV/366 nm | Rf | Visible | UV/366 nm | Rf | Visible | UV/366 nm | Rf | Visible | ||
| AF | 0.00 | Brown | Green | 0.00 | Brown | Yellow | 0.00 | Brown | Green | 0.00 | Yellow | Brown | 0.00 | Grey | Coumarins A (Guy et al. 2010), B (Mamyrbekova-Bekro et al. 2013a, b), Flavonoids C (Kabran et al. 2011; Guy et al. 2010); Tannins D (Mamyrbekova-Bekro et al. 2013a; Kabran et al. 2011) |
| 0.04 | – | Purple | – | – | – | 0.03 | Yellow | Violet | – | – | – | 0.04 | Grey | Tannins D (Mamyrbekova-Bekro et al. 2013a; Kabran et al. 2011) | |
| 0.12 | Yellow | Orange | 0.12 | – | Orange | 0.14 | Yellow | Orange | – | – | – | – | – | Coumarins B (Mamyrbekova-Bekro et al. 2013a; Kabran et al. 2011), Xanthone a (Ladyguina et al. 1983), | |
| 0.16 | – | Blue | 0.17 | Yellow | Green | – | – | – | 0.16 | Yellow | Blue | – | – | Phenolic acids or catechols a, C (Guy et al. 2010; Dawson et al. 1991) | |
| 0.91 | – | Purple | – | – | – | 0.98 | – | Purple | 0.91 | – | Violet | – | – | Hydroxyl flavonols a (Dawson et al. 1991) | |
Mobile phase : AcOEt: CHCl3: AcOH (8: 7: 0.5 (v/v/v))
l Light, d Dark, g Greenish, f Fluorescent