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Fully factorial design consisting of 12 sets for the study of 3 experimental factors in coded units was evaluated using Design Expert 8 software
| Set | Nisin(IU/ml) | Lysozyme(U/ml) | EDTA(mM) |
|---|---|---|---|
| 1 | 7,500 | 5,000 | 20 |
| 2 | 7,500 | 5,000 | 10 |
| 3 | 7,500 | 1,000 | 20 |
| 4 | 7,500 | 1,000 | 10 |
| 5 | 5,000 | 5,000 | 20 |
| 6 | 5,000 | 5,000 | 10 |
| 7 | 5,000 | 1,000 | 20 |
| 8 | 5,000 | 1,000 | 10 |
| 9 | 2,500 | 5,000 | 20 |
| 10 | 2,500 | 5,000 | 10 |
| 11 | 2,500 | 1,000 | 20 |
| 12 | 2,500 | 1,000 | 10 |
