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. 2014 Jun 24;52(6):3837–3842. doi: 10.1007/s13197-014-1445-0

Table 2.

Qualitative analysis of amino acid using thin layer chromatography

Distance travelled by Solvent (cm) Colour of the spot Rf Value of standard Calculated Rf value Amino acid
0.40 Agaricus bisporus
8.8 Pink 0.59 0.51 Methionine
8.8 Blue 0.16 0.17 Arginine
8.8 Brown 0.65 0.65 Tryptophan
8.8 Pink 0.25 0.23 Threonine
8.8 Pink 0.35 0.39 Valine
8.8 Pink 0.33 0.34 Isoleucine
8.8 Brown pink 0.50 0.53 Phenylanine
8.8 Yellow 0.19 0.21 Lysine
8.8 Orange 0.22 0.23 Histidine