Table 5.
ANOVA of central composite design model for chitin isolation
| Sum of square | Degrees of freedom | Mean square | F value | |
|---|---|---|---|---|
| (a) ANOVA for demineralized of wet shrimp byproduct | ||||
| X1 (Linier, L) | 7293.14 | 1 | 7293.140 | 147.4282a |
| X1 (Quadratic, Q) | 2775.86 | 1 | 2775.855 | 56.1129a |
| X3 (L) | 1912.78 | 1 | 1912.779 | 38.6661a |
| X3 (Q) | 188.37 | 1 | 188.369 | 3.8078 |
| X1 × X3 | 55.66 | 1 | 55.662 | 1.1252 |
| Lack of Fit | 545.91 | 3 | 181.970 | 3.6785 |
| Pure error | 148.41 | 3 | 49.469 | |
| Total SS | 12742.34 | 11 | ||
| (b) ANOVA for demineralized of dry shrimp byproduct | ||||
| X1 (L) | 4471.10 | 1 | 4471.098 | 1577.367a |
| X1 (Q) | 573.07 | 1 | 573.066 | 202.173a |
| X3 (L) | 2322.55 | 1 | 2322.546 | 819.376a |
| X3 (Q) | 74.53 | 1 | 74.533 | 26.295a |
| X1 × X3 | 1868.77 | 1 | 1868.773 | 659.288a |
| Lack of Fit | 176.60 | 3 | 58.868 | 20.768a |
| Pure error | 8.50 | 3 | 2.835 | |
| Total sum of square | 10522.66 | 1 | ||
| (c) ANOVA for deproteinization of demineralized shrimp byproduct | ||||
| X1 (L) | 3131.451 | 1 | 3131.451 | 17872.24a |
| X1 (Q) | 634.061 | 1 | 634.061 | 3618.80 a |
| X3 (L) | 1844.774 | 1 | 1844.774 | 10528.74a |
| X3 (Q) | 436.890 | 1 | 436.890 | 2493.48a |
| X4 (L) | 404.373 | 1 | 404.373 | 2307.89a |
| X4 (Q) | 7.538 | 1 | 7.538 | 43.02a |
| X1 × X3 | 137.432 | 1 | 137.432 | 784.37a |
| X1 × X4 | 36.121 | 1 | 36.121 | 206.16a |
| X3 × X4 | 24.146 | 1 | 24.146 | 137.81a |
| Lack of Fit | 236.924 | 5 | 47.385 | 270.44a |
| Pure Error | 0.526 | 3 | 0.175 | |
| Total SS | 6734.800 | 17 | ||
a = significant
a and b: X1, concentration of HCl solution (%, v/v); X2, reaction time (h); X3, solid liquid ratio of HCl solution (w/v) and X4, number of treatments. c: X1, Concentration of NaOH solution (%, w/v); X2, reaction time (h); X3, reaction temperature (°C); X4, solid liquid ratio of NaOH solution (w/v); X5, number of treatments