Figure 1.

The Δtlp mutants that are defective in chemotaxis toward essential nutrients. Chemotaxis of tlp10 (A) and tlp6 (B) was assessed by determining the relative chemotactic ratio (RCR) which is the ratio of bacterial numbers migrating toward the chemical in the syringe to the bacterial numbers migrating toward the chemotaxis buffer. Chemotaxis was determined using the capillary method and significant results were further confirmed using the disc diffusion method (Table S3). Mucin and a ΔcheY mutant were used as a positive and negative control, respectively. An RCR-value of 2 or above indicates chemotaxis toward the test chemical. Only those Δtlp mutants with a significant defect in chemotaxis are shown and the complete chemotaxis results for all compounds tested are listed in Table 2. The data represent the average and SE of three experiments with 3 replicates in each experiment. ^*P ≤ 0.05, ^**P ≤ 0.01.