Table 1.
Major and relevant findings on C. jejuni Tlps from previous studies.
| Tlp protein | Protein designation | Ligand | Mutant phenotype | This study (C. jejuni 81-176) | ||
|---|---|---|---|---|---|---|
| Motility | In vivo colonization of chickens | Other | ||||
| Tlp4 - 81-176 (Hendrixson and Dirita, 2004) - NCTC11168 (Vegge et al., 2009) -NCTC11168-O (Li et al., 2014) | Tlp4 (DocC) | - Sodium deoxycholate (Li et al., 2014) - No chemotaxis defect (Vegge et al., 2009) | - Decreased (Vegge et al., 2009) | - Decreased (Hendrixson and Dirita, 2004) | - Decreased invasion (Human Colo 205 cells, chicken embryo intestinal cells) | - No significant deficiency in the phenotypes was observed |
| Tlp5 - 81-176 (Hendrixson et al., 2001) | Tlp5 | - Unknown | - Mutant could not be generated | - Mutant could not be generated | - Mutant could not be generated | - Mutant could not be generated |
| Tlp6 - 81-176 (Hendrixson and Dirita, 2004) | Tlp6 | - Unknown | - Unknown | - No effect (cecum) | - Unknown | Decreased chemotaxis toward aspartate, isocitrate, succinate, and propionate - Decreased colonization of the chicken duodenum and jejunum |
| Tlp8 - NCTC11168 (Reuter and van Vliet, 2013) | CetZ | - Unknown (Energy taxis) | - No effect | No effect | - Opposing role in energy taxis | - Increased motility - Decreased biofilm formation - Decreased colonization of the chicken duodenum and jejunum - Decreased invasion in INT 407 cells |
| Tlp9 - NCTC11168 (Vegge et al., 2009; Reuter and van Vliet, 2013) - 81-176 (Hendrixson et al., 2001) | CetA | - Energy taxis - Decreased chemotaxis toward pyruvate | - Decreased (Vegge et al., 2009; Reuter and van Vliet, 2013) - Decreased in defined medium (Hendrixson et al., 2001) | - No effect | - Unknown | - Decreased invasion in INT 407 cells |
| Tlp10 - 81-176 (Hendrixson and Dirita, 2004) - NCTC11168 (Vegge et al., 2009) | DocB | - Unknown | - No effect | - Decreased in ceca and in proximal and distal intestinal tract | - Decreased invasion (NCTC11168 in Colo 205 cells, chicken embryo intestinal cells) (Vegge et al., 2009) | - Decreased chemotaxis toward aspartate and fumarate -Colonization defect in the chicken cecum, duodenum and jejunum |