Table 2.
RCR-values for the WT and chemotaxis mutants for all compounds tested.
| Compound | WT | Δtlp4 | Δtlp6 | Δtlp8 | Δtlp9 | Δtlp10 |
|---|---|---|---|---|---|---|
| Average CFU | Average CFU | Average CFU | Average CFU | Average CFU | Average CFU | |
| (RCR)a | (RCR) | (RCR) | (RCR) | (RCR) | (RCR) | |
| Aspartate | (9.76 ± 0.85)*105 (3.81 ± 0.3) | (2.21 ± 0.63)*106 (8.65 ± 2.46) | (1.74 ± 0.59)*105 (0.68 ± 0.23) | (6.17 ± 1.56)*105 (2.41 ± 0.61) | (8.69 ± 3.33)*105 (3.39 ± 1.3) | (2.30± 1.05)*105 (0.90 ± 0.41) |
| L-glutamine | (3.79 ±0.48)*106 (4.19 ± 0.5) | (1.26 ± 0.14)*107 (13.98 ± 1.48) | (1.64 ± 0.44)*106 (1.81 ± 0.48) | (3.13 ± 0.84)*106 (3.46 ± 0.93) | (3.3 ± 0.78)*106 (3.65 ± 0.87) | (1.56 ± 0.17)*106 (1.73 ± 0.19) |
| L-serine | (1.21± 0.23)*106 (4.03 ± 0.76) | (2.29 ± 0.23)*106 (7.62 ± 0.75) | (1.6± 0.64)*106 (5.32 ± 2.12) | (1.33 ± 0.36)*106 (4.42 ± 1.21) | (1.15 ± 0.32)*106 (3.82 ± 1.08) | (1.19 ± 0.52)*106 (3.95 ± 1.73) |
| Fumarate | (2.47 ±0.58)*106 (5.48 ± 1.28) | (4.64 ± 0.58)*106 (10.32 ± 1.29) | (1.44 ± 0.34)*106 (3.19 ± 0.76) | (1.31 ± 0.14)*106 (2.90 ± 0.31) | (1.89 ± 0.65)*106 (4.21 ± 1.44) | (5.09 ± 2.16)*105 (1.13 ± 0.48) |
| Isocitrate | (7.05 ± 1.15)*105 (2.51 ± 0.41) | (1.17 ± 0.64)*106 (4.17 ± 2.28) | (6.18 ± 3.65)*104 (0.22 ± 0.13) | (1.43 ± 0.45)*106 (5.08 ± 1.59) | (1.59 ± 0.33)*106 (5.65 ± 1.18) | (7.56 ± 2.05) *105 (2.69 ± 0.73) |
| Formate | (6.02 ±0.18)*106 (4.41 ± 0.13) | (8.43 ± 2.57)*106 (6.18 ± 1.88) | (3.29 ± 1.24)*106 (2.41 ± 0.91) | (3.11 ± 0.93)*106 (2.28 ± 0.68) | (6.55 ± 1.66)*106 (4.8 ± 1.22) | (2.32 ± 0.34)*106 (1.7 ± 0.25) |
| Succinate | (1.95 ±0.13)*106 (4.68 ± 0.32) | (3.68 ± 2.32)*106 (8.81 ± 5.56) | (4.88 ± 1.96)*105 (1.17 ± 0.47) | (2.29 ± 0.65)*106 (5.48 ± 1.56) | (2.14 ± 1.75)*106 (5.13 ± 4.18) | (7.81 ± 2.51)*105 (1.87 ± 0.60) |
| Pyruvate | (1.85 ± 0.04)*106 (3.46 ± 0.08) | (2.95 ± 0.21)*106 (5.52 ± 0.39) | (1.07± 0.18)*106 (2.01 ± 0.32) | (1.13± 0.34)*106 (2.11 ± 0.63) | (6.95 ± 0.59)*105 (1.3 ± 0.11) | (1.27 ± 0.3)*106 (2.37 ± 0.55) |
| Propionate | (4.04 ± 1.47)*106 (3.33 ± 1.21) | (3.86 ± 0.17)*106 (3.19 ± 0.14) | (1.14 ± 0.45)*106 (0.94 ± 0.37) | (6.8 ± 2.33)*106 (5.6 ± 1.92) | (6.03 ± 1.15)*106 (4.97 ± 0.95) | (4.38 ± 3.64)*106 (3.61±3) |
a
The RCR was calculated by taking the ratio of bacterial numbers migrating toward the chemical in the syringe to the bacterial numbers migrating toward the chemotaxis buffer. The results show the means of three independent assays. An RCR-value of 2 and above indicates chemotaxis toward the test chemical (Mazumder et al., 1999). RCR-values that were significantly lower than 2 (P < 0.05) are indicated in bold font.