Figure 3. Thermodynamics of mutant protein heterocomplex formation.

ITC data of (A) WD4_C1A2 titrated with wild type Cu⁺-Atox1 and (B) Atox1_C1A2 titrated with wild type Cu⁺-WD4. Thermodynamic parameters obtained from fitting of the binding isotherms in A and B are reported in Table 1.