Figure 5.
Ultrastructural analysis of retinas transduced with RHO-2/8 and RHO-2/rh10 in Rho–/– mice. Eyes of Rho–/– mice were subretinally injected with 5 × 109 vg/eye of either (b and e) AAV2/8-BB24 (RHO-2/8) or (c and f) AAV2/rh10-BB24 (RHO-2/rh10) at p3-4. Both AAVs were mixed with 1 × 108 vg/eye of AAV2/5-EGFP tracer; (a and d) untreated eyes served as controls. Eyes were fixed and retinas were whole mounted 6 months postinjection. Transduced areas of the retinas were identified by EGFP fluorescence, and representative areas were excised, postfixed, and further processed for transmission electron microscopy. Semi- and ultrathin sections were analyzed by light (a–c) and transmission electron microscopy (d–f), respectively. GCL, ganglion cell layer; INL, inner nuclear layer; IS, rod inner segment; ISL, inner segment layer; OS, rod outer segment; OSL, outer segment layer; RPE, retinal pigment epithelium; *photoreceptor segments; ◆, RPE/INL boundary; scale bar (panel c): 25 μm; scale bar (panel f): 1 μm.