Figure 6.

Sequence alignments and homology model of psysol 2 (1). (A) The amino acid sequence of psysol 2 (1) was used for alignment based on the conserved CCK motif with the prototypic cyclotides kalata B1 (Möbius type), cycloviolacin O2 (bracelet), and MCoTI-1 (cyclic TI). The structural model of the novel cyclotide psysol 2 (1) was obtained with the CycloMod tool (www.cybase.org), and the structural features were compared to kalata B1 (pdb code: 1NB1), cycloviolacin O2 (2KNM), and MCoTI-1 (1IB9). All structures are presented as ribbon cartoons, which were prepared using PyMol. β-sheet motifs are indicated by flat arrows, and the disulfide bonds are highlighted in yellow.