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. 2015 May 6;2:15013. doi: 10.1038/mtm.2015.13

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Copyright © 2015 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Overexpression of proNGF does not exert proinflammatory effect in EC. (a–c) Representative western blots statistical analysis of proNGF and TNF-α of concentrated EC-supernatants normalized to BSA-IgG. (d) Statistical analysis of mRNA expression of TNF-α normlaized to 18S. 2 × 2 analysis showed no effect of proNGF overexpression or silencing p75^NTR on altering levels of TNF-α expression in EC lysates or released proNGF or TNF-α into supernatant of EC cultures. Results presented as mean ± SD. n = 4 per group.