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. 2015 Apr 8;2:15010. doi: 10.1038/mtm.2015.10

Figure 1.

Figure 1

Schematic representation of the rAAV2/8-RSV-GFP genome and position of PCR primers. The expression cassette contains the complete Rous Sarcoma Virus (RSV) LTR promoter followed by an artificial pCI intron from the pCI plasmid backbone (Promega, Accession Number U47119), the GFP transgene, and the late poly-Adenylation signal from the SV40 virus. The bottom panel represents the two CpG Rich Region CRR1 and CRR2 identified in the RSV. CpG positions are marked by black vertical lines and numbered from 1 to 13 for CRR1, and from 1 to 9 for CRR2. Position of qPCR primers and bisulfite/PCR primers are represented above and below the figure, respectively.