Figure 3.

Persistence of AAV vector genome in dystrophic muscles and its implication on the reduction of transgene expression. mdx4Cv mice and C57BL/6J (B6) controls received bilateral intramuscular injection of an rAAV2/8-RSV-GFP vector (AAV, 4 × 10¹² vg/kg) or dPBS (Vehicle) in TA and were sacrificed 15 or 60 days postinjection. (a) Quantification of AAV vector genome per diploid genome (vg/dg) by Taqman qPCR against GFP normalized with Albumin. (b) Plotting of individual ratios between GFP mRNA RQ and the AAV vg/dg copy number. The data represent the median value (central line), the first and third quartiles (box) and first and ninth deciles (whiskers) of 10 mice per group. The shaded area delineates qPCR limit of detection. Statistics: Mann–Whitney’s U-test; *P < 0.05; **P < 0.01; ***P < 0.001; n.s., non significant.