Table 1. Quantification of dysferlin western-blot performed with Hamlet and Romeo antibodies and expressed in percentage compared to WT.
WT | NI | Set1 | Set2 | Set3 | fAAV | |
---|---|---|---|---|---|---|
N-ter Romeo Ab | 100% | 5% | 27% | 40% | 664% | 60% |
SEM | ± 0 | ± 1.3% | ± 10.3 | ± 8.4 | ± 228% | ± 27.5% |
C-ter Hamlet Ab | 100% | 1.34% | 28.74% | 17.76% | 378.83% | 41.67% |
SEM | ± 0 | ± 0.7% | ± 11.3% | ± 3.1% | ± 128% | ± 23.8% |
The blots were performed in duplicate with n = 4.
SEM, standard error of the mean.