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. 2015 May 27;5:10420. doi: 10.1038/srep10420

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(A): PC-3M cells were pretreated with CHX (25 μM) and then further treated with 50 μM curcumin for 24 h; then, phase-contrast images were observed. (B): Western blots showing the effect of CHX on the curcumin-induced expression of LC3 and ubiquitinated proteins. (C): Phase-contrast images showing the effect of LC3 knockdown on curcumin-induced cytoplasmic vacuolation. Vacuolated PC-3M cells are indicated by yellow arrows. (D): Western blots of the total cell lysates showing the effect of LC3 knockdown on the curcumin-induced expression of LC3 and ubiquitinated proteins.