Figure 2.

Characterization of tumor-derived exosomes by electron microscopy and western blot analysis. A and B: Exosomes released by SGC/wt or SGC/kd cells were isolated and observed with an electron microscope. The exosomes are small round vesicles limited by a lipid bilayer, and their diameters are between 30 and 100 nm. Scale bars are as indicated; C: Western blot analysis of exosomal lysate proteins. The published exosomal markers, Hsp70 and CD9, were detected in exosomes derived from gastric cancer cells, indicating successful exosome isolation. SGC/wt-exo: Exosomes isolated from SGC/wt cells; SGC/kd-exo: Exosomes isolated from SGC/kd cells.