Figure 3.

SGC/wt cells-derived exosomes promoted tumor cell proliferation and invasion in vitro. A: A total of 5 × 10³/well SGC/wt and SGC/kd cells were seeded in 96-well plates in RPMI 1640 medium without fetal bovine serum (FBS). After incubating overnight, the cells were treated with wt-exo or kd-exo at the concentrations of 0, 50, 100, 200 and 400 μg/mL for 24 h, and cell proliferation was measured by MTS assay. The results showed that wt-exo significantly promoted proliferation of both SGC/wt and SGC/kd cells in a dose-dependent manner. ^aP < 0.05, ^bP < 0.01 vs kd-exo group; B: SGC/wt and SGC/kd cells were treated with 200 μg/mL of indicated exosomes for 4 h, and seeded on Matrigel matrix coated chambers for the invasion assay; C: Invaded cell counting revealed that the number of penetrated cells in the wt-exo group was significantly higher than that in the kd-exo group. ^bP < 0.01 vs kd-exo group. Representative crystal violet staining after 36 h invasion showed invaded cells in each group. Data are expressed as mean ± SD. wt-exo: SGC/wt-derived exosomes; kd-exo: SGC/kd-derived exosomes.